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Title   인체 간암조직에서 라우스 육종 바이러스 Engancer Factor 유전자의 발현 ( Expression of Rous Sarcoma Virus Engancer Factor Gene in Human Hepatocellular Carcinoma )
Publicationinfo   1997 Jan; 029(04): 547-555.
Key_word   RSV, LTRs, Hepatocellular carcinoma, Enhancer factor, Y-box
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Abstract   Purpose: We have previously cloned three enhancer factor genes encoding proteins that bind to long terminal repeats(LTRs) of Rous sarcoma virus. Among these genes, RSV-EF-I gene is expressed in rat hepatoma tissues and several proliferating cell lines but not in normal rat liver tissues. We have isolated the human homologue of RSV-EF-I gene and examined its expression in human hepatocellular carcinoma tissues. Materials and Methods: We have screened the human genomic library and cDNA library of Hep G2 cell line derived from human hepatocellular carcinoma to isolate the human homologue of RSV-EF-I gene. Results: We have isolated one cDNA clone containing about 1.5 kb insert and sequenced. Sequence analysis reveals that this human homologue of RSV-EF-I gene has a high similarities to human YB-1 mRNA, human DNA-binding protein B(dbpB) gene and other Y-box protein genes. It is expressed in human hepatocellular carcinoma but very slightly in normal human liver tissues in Northern blot analysis. Conclusion: Our data suggest that the human homologue of RSV-EF-I gene presumably belongs to Y-box protein family genes and plays a role in the transformation of the human hepatoma cells.
저 자   신부안(Boo An Shin),김경근(Kyung Keun Kim),조철균(Chul Kyun Cho),이현철(Hyun Chul Lee),오종석(Jong Seok Oh),이준행(Joon Haeng Lee),장미영(Mi Young Jang)